As a N2-fixing tree species, sea buckthorn (Hippophae rhamnoides) is well adapted to arid regions and is utilized for multiple purposes in China. Current knowledge of genetic variability of H. rhamnoides is limited in terms of rangewide distributions. Eleven natural populations of sea buckthorn in northeastern and northwestern China were analyzed to detect genetic variation among and within populations, by use of ISSR (inter-simple sequence repeats) markers. Using eight primers, 207 polymorphic loci were observed, ranging in size from 250 to 2500 bp. The coefficient of gene differentiation (Gst = 0.0679) showed that the total molecular variance of 11 populations was mainly existed within populations. The genetic variation within and among the 11 populations was 93.21 and 6.79%, respectively. No significant correlation between genetic and geographic distances of the populations was found using ISSR markers. Our study provides a population-level genetic profile for further investigation and conservation of genetic diversity of sea buckthorn. 相似文献
A mangrove stand of Avicennia marina located at the South Corniche of Jeddah city receives about 100 000 m3 of sewage per day. The sewage has high values of BOD, COD, nutrient concentrations, heavy metals and faecal coliform counts.The mangrove stand is very small and occupies an area of about 0.45 km2. The trees are stunted with low pneumatophore density. The pneumatophore distribution is restricted to the area of substrate underneath the plant crown covers and they do not look healthy. A high proportion of them was observed to be dead and/or aberrant. These abnormal induced features were attributed to the sewage discharge in the area. The death of pneumatophores decreases the aeration area which apparently affects the respiration rate of the root system, nutrient uptake and plant growth, consequently leading to a retarded growth of the mangroves. 相似文献
The safety and efficacy of emamectin benzoate, administered in-feed to Atlantic salmon smolts, Salmo salar L., held in freshwater, was evaluated as a preventative treatment against sea lice, Lepeophtheirus salmonis, following transfer of fish to seawater.
In the safety study, salmon smolts held in freshwater were fed with diets containing emamectin benzoate at nominal doses of 0 (control), 50 (recommended dose) and 250 (5× recommended dose) μg kg−1 fish day−1 for 7 days (days 0–6). Actual dose rates, based on measured concentrations of emamectin benzoate in feed, differences in fish weight, and feed consumed, were 0, 54, and 272 μg kg−1 day−1, respectively. On day 9, fish were transferred to seawater and observed for 14 days. No differences in feeding response, coordination, behaviour, gross and histological appearance were observed between control fish and those that received 54 μg kg−1 day−1. Among smolts that received 272 μg kg−1 day−1, approximately 50% exhibited darker coloration, and one fish (1%) exhibited uncoordinated swimming behaviour. No pathognomonic signs of emamectin benzoate toxicity were identified.
In the efficacy study, smolts held in freshwater were fed an unmedicated ration (control group) or emamectin benzoate at 50 μg kg−1 day−1 (treated group) for 7 days (days 0–6). On day 9, fish were re-distributed to eight seawater tanks, each holding 30 control and 30 treated fish. On days 28, 56, 77 and 109, respectively, control and treated fish in two tanks were challenged with L. salmonis copepodites. When lice in each group reached chalimus stage IV, fish were sampled and the numbers of lice were recorded. Fish challenged at day 109 were sampled for the second time when lice were at the adult stage. Efficacy was calculated as the reduction in the mean number of lice on treated fish relative to the mean on control fish. Treatment with emamectin benzoate resulted in an efficacy of 85.0–99.8% in fish challenged at days 28–77, from the start of treatment, and lice counts were significantly lower (P<0.001) on treated fish than on controls. When fish challenged at day 109 were sampled at day 128, efficacy was 44.3%, but survival of chalimus to adult lice on treated fish was lower, and at day 159, efficacy had increased to 73%. These results demonstrate that treatment of salmon smolts with emamectin benzoate in freshwater was well tolerated and highly effective in preventing sea lice infestation following transfer of fish to seawater. 相似文献
Following independence from Ethiopia in 1993, Eritrea resumed exploiting Red Sea and Arabian fish species for the ornamental trade in 1995 as a means to earn foreign exchange from sparsely exploited marine resources. This paper describes the findings of research conducted in 1997, in collaboration with the Eritrean Ministry of Fisheries. The capture, transport and export of aquarium fish were reviewed and potential impacts and the status of management were investigated through liaison with stakeholders and researchers. From 1995 to 1997 two companies exported approximately 60,000 fish per year, mainly to the USA, worth US$65,000 (export value). Seventy-five species (from 22 families) were exported. Damselfishes made up two-thirds of total exports but more valuable families (angelfishes and butterflyfishes) were more economically significant. To earn revenue for Eritrea, a 20% export tax was imposed, although this was calculated from declarations by the operators. The emerging nature of the trade allowed detailed monitoring by the Ministry of Fisheries. However, management efforts were constrained by a lack of capacity for enforcement and baseline research. Several potential effects of the trade exist but other, land-based impacts may be more pressing concerns for Eritrea's reefs. Research priorities for management are discussed as well as the implications of mariculture of Eritrean species by other nations. 相似文献